Divine Aleru, Microbiome Signatures Research Coordinator

This study identifies phospho-2-dehydro-3-deoxyheptonate aldolase as a drug target in Gardnerella vaginalis and highlights five DA-approved compounds as potential treatments for bacterial vaginosis.

This study explores the link between vaginal microbiota and recurrent spontaneous abortion (SA), showing that drug treatment, including metformin combined with aspirin, can alter microbiota composition and potentially improve pregnancy outcomes by increasing Lactobacillus abundance.

This study identifies AHP synthase as a promising drug target for Gardnerella vaginalis in the treatment of bacterial vaginosis, presenting potential inhibitors with favorable pharmacokinetics.

BD shows antibacterial and antibiofilm activity against Gardnerella vaginalis, suggesting its potential as a novel treatment for bacterial vaginosis, particularly in biofilm-related infections.

The study analyzed the genetic evolution of Gardnerella vaginalis, focusing on its resistance and virulence under Darwinian positive selection. It identifies new drug targets and emphasizes the pathogen’s evolving resistance mechanisms.

V disrupts Lactobacillus dominance, increasing infertility risk via inflammation, ID, and endometritis. L. crispatus probiotics reduce V recurrence; E treatment boosts VF success. Early screening and microbiome-targeted therapies are vital to prevent reproductive complications.

Study links V and past chlamydial infection to tubal infertility, with 87.5% of V-positive women having tubal damage. Both infections were often asymptomatic. VF pregnancy rates were unaffected, but V showed lower implantation trends. Findings highlight V’s role in infertility, urging early screening to prevent tubal damage.

This review links V recurrence to resilient biofilms formed by Gardnerella vaginalis. Probiotics and biofilm disruptors (e.g., Astodrimer gel) improve outcomes by restoring Lactobacillus dominance. Current antibiotics fail to penetrate biofilms, necessitating multimodal therapies. Future research should explore MT and microbiome-targeted interventions for sustained V remission.

This study links high pre-treatment vaginal microbiota diversity to V recurrence after metronidazole. Women with sustained clearance had lower richness. Lactobacillus iners improved immune markers temporarily, but no cases achieved L. crispatus dominance. Biofilm-forming taxa like Atopobium persisted, suggesting resistance mechanisms.

This review reveals high V recurrence rates after metronidazole or clindamycin treatment due to microbial biofilms and potential sexual transmission. While both antibiotics show similar short-term efficacy, they differ in resistance patterns. Biofilm disruptors and partner treatment may improve outcomes, but better diagnostics and combination therapies are urgently needed.

This meta-analysis compared V treatments, identifying ornidazole as the most effective oral drug and sucrose/probiotics as top non-antibiotic options. Restoring Lactobacillus dominance is key, with vaginal probiotics and sucrose showing high cure rates.

This review explores the role of vaginal microbiota in bacterial vaginosis and highlights emerging microbiome-informed treatments. It emphasizes microbial signatures of V, the limitations of antibiotics, and the potential of targeted biotherapeutics to restore microbial balance and reduce recurrence.

This systematic review analyzed six trials evaluating secnidazole for bacterial vaginosis treatment. Secnidazole at 2 g significantly improved clinical and microbiologic cure rates, showing comparable efficacy to metronidazole. The single-dose regimen enhances adherence, offering an alternative for patients with recurrent V or adverse effects from standard therapies.

This meta-analysis reviewed 12 randomized controlled trials and found that probiotics significantly improve the clinical cure rate of bacterial vaginosis. Probiotic use restored beneficial Lactobacillus populations and reduced pathogenic bacteria, highlighting their potential as an effective treatment option and adjunct therapy alongside antibiotics.

This study evaluated a Saccharomyces cerevisiae-based probiotic for bacterial vaginosis treatment. The probiotic selectively inhibited V-associated pathogens like G. vaginalis without harming beneficial lactobacilli, offering a microbiome-friendly alternative to antibiotics.

This cross-sectional study identified a strong link between elevated serum lead and cadmium levels and increased risk of bacterial vaginosis. It suggests that heavy metal exposure may disrupt vaginal microbiota stability and immunity, contributing to V susceptibility and pointing to new environmental factors in V prevention strategies.

This study revealed how bacterial vaginosis alters vaginal metabolism, linking specific microbial shifts with distinctive metabolite profiles. It showed that V-associated bacteria drive metabolic changes that increase vaginal pH, disrupt epithelial integrity, and produce characteristic symptoms, offering new diagnostic markers and therapeutic targets.

This review explains how microbial shifts, metabolite production, and immune responses interact in bacterial vaginosis. It highlights the roles of Gardnerella, Atopobium, and other anaerobes in disrupting vaginal health and discusses how their metabolic byproducts and immune modulation drive V symptoms and persistence.

This study used a multi-omic approach to reveal key microbial and metabolic markers of bacterial vaginosis. It identified two distinct symptomatic V metabotypes, each defined by unique microbial and metabolite associations, underscoring the need for metabolomics-informed clinical diagnostics.

This study highlights metabolic interactions between Gardnerella vaginalis and Prevotella bivia, revealing their role in bacterial vaginosis and preterm birth risk. Using MR metabolomics, it identifies acetate as a key metabolite linked to vaginal dysbiosis, offering insights for improving V diagnostics and potential microbiome-targeted interventions.

A nutrient-rich diet lowers bacterial vaginosis (V) risk, while processed foods increase it. This study highlights the importance of dietary choices in vaginal microbiome balance, offering insights for clinicians on how diet influences V risk.

A high glycemic load diet increases bacterial vaginosis (V) risk, while nutrient-rich diets lower it. This study highlights the link between diet quality and vaginal microbiome balance, providing insights for clinicians on how dietary interventions may help prevent V.

A diet high in processed foods and sugar increases bacterial vaginosis (V) risk, while a plant-based diet lowers it. This study highlights the importance of dietary choices in vaginal health, providing insights for clinicians on how nutrition influences the vaginal microbiome.

Higher dietary fat intake increases the risk of bacterial vaginosis (V), while folate, vitamin E, and calcium may lower severe V risk. This study suggests dietary interventions could play a role in managing V, offering insights for clinicians on how nutrition affects vaginal microbiome balance.

The Nugent Score is a standardized Gram stain-based scoring system used to diagnose bacterial vaginosis (V) by assessing key bacterial morphotypes in vaginal samples. With its high specificity, it remains a gold standard in microbiome research, though its complexity and need for trained personnel make it less common in routine clinical practice.

Amsel’s Criteria is a clinically established diagnostic method for bacterial vaginosis, offering a practical and accessible alternative to laboratory-based approaches. It evaluates four key clinical indicators, ensuring timely diagnosis and intervention in outpatient settings.

This study analyzed daily changes in vaginal microbiota before, during, and after V episodes. Findings highlight microbial instability, early dysbiosis markers, and the need for microbiome-focused V treatments.

This study assessed vaginal microbiota in V-positive women using real-time CR. It found reduced Lactobacillus crispatus, biofilm-associated pathogens, and high diagnostic accuracy for molecular testing. Results suggest CR-based diagnostics improve V detection and highlight the need for microbiome-targeted therapies.

This study analyzed vaginal microbiota diversity in women with V using molecular techniques. It identified key V-associated bacteria and emphasized the need for molecular diagnostics and microbiome-targeted therapies.

This study examines vaginal microbiome recovery after metronidazole treatment for V. Findings show delayed microbiota stabilization, L. iners dominance post-treatment, and the need for improved diagnostic tools to predict V recurrence.

This study used high-resolution sequencing to analyze bacterial communities in women with V. Findings reveal distinct microbial associations, diagnostic inconsistencies, and racial differences in V prevalence, highlighting the need for microbiome-targeted therapies and improved diagnostic tools.

This review explores bacterial vaginosis, focusing on microbial imbalances, immune responses, and diagnostic challenges. It highlights the need for microbiome-based treatments and improved diagnostics to reduce recurrence and improve V management.

This study identifies bacterial vaginosis-associated species, improving V diagnostics and treatment strategies through metagenomic sequencing.

This review explores bacterial vaginosis, emphasizing its recurrence, microbial associations, and treatment challenges. It highlights the need for microbiome-based therapies, standardized diagnostic criteria, and potential partner treatment to reduce reinfection. The findings underscore the importance of improved strategies for long-term V management.

This review explores bacterial vaginosis recurrence, emphasizing microbial persistence, reinfection, and partner treatment. It highlights the need for improved therapeutic strategies, including antimicrobial and microbiome-focused approaches, to reduce recurrence and improve long-term cure rates.

This review explores the competing hypotheses behind bacterial vaginosis (V), emphasizing microbial shifts, racial disparities, and sexual transmission. Key findings suggest V may be sexually transmitted and driven by Gardnerella vaginalis biofilms, necessitating novel treatment strategies beyond standard antibiotics. Understanding V pathogenesis is essential for improving prevention and care.

This review explores bacterial vaginosis (V), emphasizing microbial shifts, diagnostic challenges, and treatment strategies. It highlights the role of biofilms, emerging molecular diagnostics, and microbiome-based therapies like probiotics and vaginal microbiota transplantation, advocating for more effective, microbiome-informed approaches to managing V and its recurrence.

This review explores bacterial vaginosis diagnostics, highlighting traditional limitations and emerging molecular solutions. Advances in sequencing, metabolomics, and I-driven analysis offer promising improvements, enhancing diagnostic accuracy and treatment strategies.

This study links vaginal microbiota composition—especially Lactobacillus iners dominance and Pseudomonadaceae enrichment—to VF outcomes in women with COS or tubal infertility, highlighting microbial markers that may predict implantation success.

This study compared gut microbiome in early-stage and late-stage endometriosis, revealing distinct microbial signatures, steroid metabolism suppression in severe disease, and a unique dysmenorrhea-associated subtype.

This study links estrogen levels to oral microbiome composition, inflammation, and viral load in OVID-19, identifying microbial signatures across hormonal groups that highlight estrogen’s protective role in maintaining oral microbial balance.

WUAP modulates gut microbiota and metabolites in a dysmenorrhea rat model, restoring Lactobacillus, reducing Romboutsia, correcting hormone-related metabolic pathways, and improving inflammatory and pain outcomes.

This study identifies distinct microbial signatures that differentiate ovarian cancer from benign conditions and reveal early-stage enrichment of pathogenic taxa with depletion in advanced disease, highlighting promising diagnostic and prognostic microbiome biomarkers for ovarian cancer.

This study shows that specific intratumoral microbiome patterns shape immunity and prognosis in ovarian cancer, identifying microbe–immune interactions and prognostic microbial signatures.

This study integrates fungal and bacterial sequencing to define pathogen-specific microbiome signatures in vulvovaginal candidiasis, revealing distinct STs shaped by C. albicans or C. glabrata and their associated bacterial communities.

This study compared gut microbiota in Japanese women with and without MS, identifying higher diversity and increased Collinsella, Bifidobacterium, and Blautia in MS, suggesting microbial signatures relevant to symptom development.

This study examined gut microbiota differences in women with premenstrual symptoms, identifying decreases in key butyrate-producing and ABA-modulating bacteria linked to symptom severity, suggesting mechanistic microbiome pathways relevant to MDs.

This study links menopausal syndrome with gut microbiome dysbiosis, identifying species correlated with estradiol and enriched metabolic pathways that may influence symptom severity and cardiometabolic risk.

This study examined the vaginal microbiome in premature ovarian insufficiency, identifying reduced Lactobacillus, increased dysbiosis-associated taxa, and strong hormone–microbe correlations that suggest a microbiome–ovary interaction.

This study examined how the premature ovarian insufficiency vaginal microbiome differs from healthy controls, identifying key microbial shifts linked to hormone disruption and ovarian dysfunction.

This study identifies distinct ovarian microbiome differences between cancerous and noncancerous tissues, showing altered taxa and enriched pathogenic and oxidative stress–tolerant functions in ovarian cancer.

This study examined vaginal microbiome signatures in women with secondary infertility, identifying Lactobacillus depletion and anaerobic enrichment, with limited microbiome response to VF-related hormonal induction.

This study compared fertile and infertile women’s gut microbiota and assessed HGG’s effects on dysbiosis and pregnancy during RT, highlighting key microbial predictors of conception.

This study links altered vaginal microbiota—especially shifts in Lactobacillus species—to hormonal disruption in primary ovarian failure, revealing microbial signatures that correlate with reproductive hormone levels and resemble menopausal microbiome patterns.

The study identifies a postpartum depressive disorder microbiome signature marked by reduced butyrate-producing bacteria and increased inflammatory taxa, correlated with depression severity and sex hormone levels.

This study identifies distinct microbial signatures in ovarian carcinoma, including increased Proteobacteria and reduced Firmicutes, with Acinetobacter lwoffii and Lactococcus piscium emerging as key taxa linked to tumor biology and diagnostic potential.

This study identifies distinct microbiome signatures in breast tissue and urine of women with breast cancer, including decreased Methylobacterium and increased gram-positive taxa, revealing potential microbial biomarkers for disease detection.