The ArsD As(III) metallochaperone Original paper
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Metals
Metals
OverviewHeavy metals play a significant and multifaceted role in the pathogenicity of microbial species. Their involvement can be viewed from two primary perspectives: the toxicity of heavy metals to microbes and the exploitation of heavy metals by microbial pathogens to establish infections and evade the host immune response. Understanding these aspects is critical for both […]
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Microbes
Microbes
Microbes, short for microorganisms, are tiny living organisms that are ubiquitous in the environment, including on and inside the human body. They play a crucial role in human health and disease, functioning within complex ecosystems in various parts of the body, such as the skin, mouth, gut, and respiratory tract. The human microbiome, which is […]
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Divine Aleru
I am a biochemist with a deep curiosity for the human microbiome and how it shapes human health, and I enjoy making microbiome science more accessible through research and writing. With 2 years experience in microbiome research, I have curated microbiome studies, analyzed microbial signatures, and now focus on interventions as a Microbiome Signatures and Interventions Research Coordinator.
Microbiome Signatures identifies and validates condition-specific microbiome shifts and interventions to accelerate clinical translation. Our multidisciplinary team supports clinicians, researchers, and innovators in turning microbiome science into actionable medicine.
I am a biochemist with a deep curiosity for the human microbiome and how it shapes human health, and I enjoy making microbiome science more accessible through research and writing. With 2 years experience in microbiome research, I have curated microbiome studies, analyzed microbial signatures, and now focus on interventions as a Microbiome Signatures and Interventions Research Coordinator.
What was studied?
This study explores the function of ArsD, a metallochaperone involved in arsenic resistance in Escherichia coli. ArsD facilitates the transfer of arsenic(III) (As(III)) to the ArsA ATPase, which is part of an ATP-driven arsenic efflux pump. This pump actively extrudes arsenic from cells, contributing to resistance against environmental arsenic. ArsD plays a critical role by increasing the affinity of ArsA for As(III), thus enhancing the efficiency of arsenic detoxification. The study investigates the crystal structure of ArsD, its interaction with ArsA, and the biochemical process by which ArsD delivers arsenic to ArsA, using X-ray crystallography and NMR spectroscopy to understand the structural and functional details of this interaction.
Who was studied?
The study focuses on the ArsD protein found in Escherichia coli, specifically examining its role in the ars operon on plasmid R773. The ArsD protein, a metallochaperone, is analyzed in relation to its interaction with ArsA, the ATPase subunit of the ArsAB pump. Various mutants of ArsD were also created to explore how changes in its structure affect its interaction with ArsA and its ability to transfer arsenic. Additionally, the study investigates the behavior of ArsD in both its free form and when bound to arsenic. It also examines ars operon sequences in other bacteria, suggesting that similar systems exist across diverse species capable of arsenic detoxification.
Most important findings
The study found that ArsD plays a critical role in arsenic resistance by enhancing the efficiency of ArsA in extruding arsenic from the cell. The cysteine residues in ArsD (Cys12, Cys13, and Cys18) form a high-affinity site for As(III), which is transferred to ArsA, thereby increasing its affinity for arsenic and improving the efflux process. The researchers also discovered that ATP hydrolysis by ArsA is required for the transfer of As(III) from ArsD to ArsA, suggesting a conformational change in ArsA during the catalytic cycle that facilitates this process. The study also demonstrated that ArsD enhances ATPase activity in ArsA, making it more effective at lower arsenic concentrations, typical of environmental conditions. Additionally, structural analysis revealed that ArsD and ArsA form a transient complex during the arsenic transfer process, with the binding sites of both proteins coming into close proximity to allow for efficient arsenic transfer.
Key implications
For clinicians, understanding the role of ArsD in arsenic detoxification provides insight into how microbial arsenic resistance mechanisms could be leveraged for bioremediation in contaminated areas. The findings suggest that ArsD’s ability to deliver As(III) to ArsA could be crucial in developing microbial treatments to clean up arsenic-contaminated water sources, a significant health concern in regions with arsenic-rich drinking water. Moreover, understanding the mechanism of action of metallochaperones like ArsD could inform the development of new therapeutic strategies for managing arsenic poisoning in humans. This could lead to more targeted approaches in environmental health, especially in arsenic-affected communities.
Arsenic can disrupt both human health and microbial ecosystems. Its impact on the gut microbiome can lead to dysbiosis, which has been linked to increased disease susceptibility and antimicrobial resistance. Arsenic's ability to interfere with cellular processes, especially through its interaction with essential metals like phosphate and zinc, exacerbates these effects. By understanding how arsenic affects microbial communities and how these interactions contribute to disease, we can develop more effective interventions, including microbiome-targeted therapies and nutritional strategies, to mitigate its harmful effects.